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Looking good on Day 3 of #Shigella2026 ☀️🇫🇷🧫

Huge thanks to Thibault Frisch, @sellinlab.bsky.social and all other co-authors and collaborators Petra Geiser, Margarita Κomi, Philip Karlsson, Anjeela Bhetwal, Laura Jenniches, @lbarquist.bsky.social , @holmqvist-lab.bsky.social , and @andrenmateus.bsky.social, for making this work possible!

This work builds on our recent Nature Genetics paper on Shigella colonization in intestinal organoids (rdcu.be/eqGgf), zooming in on one of the screen hits: pcnB.

A scalable gut epithelial organoid model reveals the genome-wide colonization landscape of a human-adapted pathogenNature Genetics - A genome-wide screen using human gut epithelial organoids combined with transposon-directed insertion sequencing identifies over 100 Shigella flexneri genes required for...rdcu.be

Thrilled to share that our study on how the pcnB gene sustains Shigella virulence is now published in PLOS Pathogens: journals.plos.org/plospathogen... In short: pcnB ➝increased virulence-plasmid replication ➝ elevated T3SS expression ➝ enhanced invasion/spread within the intestinal epithelium.

The pcnB gene sustains Shigella flexneri virulenceAuthor summary Bacterial infections represent a major global threat. Understanding the genetic determinants promoting infections is crucial to overcome this threat. Shigella is an intracellular bacter...journals.plos.org

Happy to announce that our paper is finally out! Thanks to @brozlab.bsky.social and co-authors @elliottbernard.bsky.social @ehartenian.bsky.social 🥳 the NLRP6 inflammasome is activated by sterile- or pathogen-induced endolysosomal damage @embojournal.org

IT'S HAPPENING! 💥 I'm psyched to launch the collaboration between @qedscience.bsky.social & @openrxiv.bsky.social @biorxivpreprint.bsky.social! Preprint + q.e.d = your science is out there, and anyone can appreciate it. Let's care about making discoveries, and not on “getting published” (1/3) 👇

Enhanced virulence and stress tolerance are signatures of epidemiologically successful Shigella sonnei @sydneylmiles.bsky.social @katholt.bsky.social 🙏to transformative collaborative effort www.nature.com/articles/s41...

So excited our antibiotic potentiation story is out 🤩 Led by the extraordinary @manonlang.bsky.social with @fox-science.bsky.social & @amazeld.bsky.social +amazing collaborators @immunobladder.bsky.social @imaneelmeouche.bsky.social 🦠 We believe it can make a difference in #AMR infections!

Uridine as a potentiator of aminoglycosides through activation of carbohydrate transportersUridine boosts aminoglycoside treatment efficiency against antibiotic-susceptible as well as antibiotic-resistant E. coli strains.www.science.org

Mast cell subsets respond uniquely to Salmonella infection. Read all about it in our latest paper in EJI. Another chapter in our deciphering of mast cell interactions with enterobacteria, driven by Chris von Beek. onlinelibrary.wiley.com/doi/10.1002/...

Mast Cell Phenotypic Heterogeneity Impacts the Interplay with Pathogenic Salmonella Typhimurium BacteriaMast cell phenotypic features influence anti-bacterial response. Connective tissue-like mast cells sense Salmonella through combined Toll-like-receptor (TLR) signaling and recognition of Type-III-sec....onlinelibrary.wiley.com

Such a rewarding collab it has been with our colleagues at #Novavax to dissect the MoA of their potent Matrix-M adjuvant. This project has really put the imaging skills of @erixoneriksson.bsky.social to the test. The fruits of this joint labour out now in NPJ Vaccines www.nature.com/articles/s41...

Matrix-M adjuvant triggers inflammasome activation and enables antigen cross-presentation through induction of lysosomal membrane permeabilization - npj Vaccinesnpj Vaccines - Matrix-M adjuvant triggers inflammasome activation and enables antigen cross-presentation through induction of lysosomal membrane permeabilizationwww.nature.com

🦠 Launching a bimonthly digest on RNA modifications and related topics in bacteria!🤩 First issue: Summer 2025. Shared here + by email, future ones will be quicker reads! 😅 #rnasky #microsky #ribosome Subscribe and share if you are interested! rnamodifupdates.substack.com/p/rna-modifi...

RNA modifications digest Summer 2025Bimonthly newsletter: RNA modifications and related topics, mostly in bacteria. Summer 2025, first issue!rnamodifupdates.substack.com

#NatMicroPicks Decoding Shigella’s Virulence Blueprint 🦠🧬 Combining organoid infection models with genome-wide mutagenesis allows mapping the full set of Shigella genes required for human gut colonization www.nature.com/articles/s41... @sellinlab.bsky.social @natgenet.nature.com

A scalable gut epithelial organoid model reveals the genome-wide colonization landscape of a human-adapted pathogen - Nature GeneticsA genome-wide screen using human gut epithelial organoids combined with transposon-directed insertion sequencing identifies over 100 Shigella flexneri genes required for epithelial colonization.www.nature.com

Could lab-grown mini-organs help scientists research a range of infections? A new study published in Nature Genetics shows how researchers have mapped how the Shigella bacterium infects the human gut. Read our interview with study co-author — and UTM Biology prof — Lars Barquist at uoft.me/bI7.

Excited to be at #EESMicrobiology this week presenting our latest work on #Shigella gut #organoid infection screen from our recent Nature Genetics paper (see below)! Stop by poster 109 or just look for me, happy to chat and connect! #Microbiology #Shigella #Organoids #TraDIS

The pcnB gene sustains Shigella flexneri virulence https://www.biorxiv.org/content/10.1101/2025.06.16.659909v1

A study in Nature Genetics establishes the comprehensive genome-wide map of Shigella genes required to infect human intestinal epithelium. go.nature.com/4e7fdKp 🧬 🧪

Huge congratulations to Thibault Frisch for leading this work to completion with remarkable insight and drive, and thanks to all co-authors for making it happen! @sellinlab.bsky.social, @lbarquist.bsky.social, @andrenmateus.bsky.social, @holmqvist-lab.bsky.social

pcnB (poly-A polymerase I) sustains replication of the Shigella virulence plasmid at levels optimal for virulence and boosts intraepithelial expansion in intestinal organoid monolayers.

New preprint out: www.biorxiv.org/content/10.1... We followed up on our recent Nature Genetics paper mapping Shigella colonization in intestinal organoids with a deep dive into one of the screen hits: pcnB. #Shigella #virulence #microsky

Thanks @sydneylmiles.bsky.social and @sergemostowylab.bsky.social for writing such a sharp and thoughtful editorial, which covers all the main points and potential caveats of our study published yesterday. www.nature.com/articles/s41...

Organoid models advance dysentery control - Nature GeneticsShigella is a major human pathogen with a stark lack of pipelines linking genome content to clinical pathogenesis. An innovative study using large-scale organoid models, combined with genome-wide muta...www.nature.com