Assistant Professor at Utrecht University 🇳🇱 We use #cryoEM and molecular #virology techniques to study the infection mechanisms of +ssRNA viruses. https://www.uu.nl/medewerkers/DLHurdiss Views are my own.
Great to see this finally published! We describe channels at the poxvirus core wall involved in nucleic acid release. www.nature.com/articles/s41...
In situ structure of the poxvirus portal complex - NatureCryo-electron tomography uncovers the structure of a conserved hexameric ‘portal complex’ that spans the core wall in vaccinia virus, and shows how it is associated with viral functions such as a...www.nature.comOne month left to apply (deadline August 31st)! 👇 #Virology #AcademicJobs #FacultyJobs #FacultyPosition #TenureTrack #GroupLeader
Daniel HurdissWe are looking for an Assistant or Associate Professor to join us at the @utrechtvirology.bsky.social. Interested in building an independent research programme in a collaborative #virology environment? Please take a look and share with anyone who might be a good fit! www.uu.nl/en/organisat...
One cell, one tomogram! Our montage tomography workflow, MOSAIC, is out in Structure, and MontageMaker is on GitHub! MOSAIC: www.cell.com/structure/fu... MontageMaker: github.com/MPI-Dortmund... Big thanks to @maikaboiero.bsky.social, Adriana Prajica, Gavin Rice, and @raunser-lab.bsky.social!
Targeting Picornavirus Nonstructural 2C Protein: Structure, Function, and Antiviral Discovery pubs.acs.org/aidcbc/artic...
Targeting Picornavirus
Nonstructural 2C Protein: Structure,
Function, and Antiviral DiscoveryAbstract. Picornaviruses cause
substantial human and veterinary disease,
yet direct-acting antivirals remain limited. The nonstructural protein
2C is amongpubs.acs.org
Thousands of possible receptor-peptide combinations. One AI-powered solution. Led by @lariferg.bsky.social, a study from @schaferlab.bsky.social & Isabel Beets has developed DeorphaNN, a neural network to identify peptide pairings for GPCRs. Read more: mrclmb.ac.uk/news-events/... #LMBResearch
Happy that our most recent paper on nucleotide excision repair mechanisms has now been published in Science Advances. This project was led by @natalia-mg.bsky.social and @callumhaste.bsky.social and focused on visualising early steps of the NER process by #cryoEM. www.science.org/doi/10.1126/...
Visualization of stepwise derepression of TFIIH in global genome nucleotide excision repairCryogenic electron microscopy captures nucleotide excision repair complexes assembling on damaged DNA templates.www.science.org
🚀 Can automation solve preferred orientation in your #cryoEM datasets? After automating processing across 21 GPCR datasets in #CryoSPARC, we updated the workflow to be even more robust to preferred orientation, achieving improved map quality over manual processing. Read more 👉 bit.ly/3RyOIGG
Direct lipid interactions control SARS-CoV-2 M protein conformational dynamics and virus assembly www.nature.com/articles/s41...
Direct lipid interactions control SARS-CoV-2 M protein conformational dynamics and virus assembly - Nature CommunicationsSARS-CoV-2 M protein binds the host lipid ceramide-1-phosphate, stabilizing its short conformation to organize viral structural proteins during assembly. Disrupting this interaction impairs virus-like...www.nature.com
Here's an interesting one: S:P812R in a BA.3.2 from Western Australia—creates a furin cleavage site (FCS) in the S2' cleavage position S:685. The classic SARS-2 FCS is at S:682-685. Spike gets cleaved by furin there before it leaves the cell (usually). The S2' cleavage site is different... 1/4
Thrilled to share our collab led by Grace Hibshman with the Shipman lab! Type IX retron-Kva2 structure reveals phage detection via an HTH fold, not a primary sequence. We computationally designed triggers that program bacteria cell death, pointing toward designer antimicrobials.
1/5: Our latest preprint is out! We show that LEA proteins can diversify particle orientations in cryo-EM, helping to overcome preferred orientation at the air-water interface (AWI). www.biorxiv.org/cgi/content/... with @cijilim.bsky.social and Tim Grant #CryoEM #LEAproteins
Diversifying particle-orientation distributions in cryo-EM with LEA protein additivesPreferential orientation at the air-water interface (AWI) remains a persistent challenge in cryo-electron microscopy, often requiring extensive optimization or specialized grid preparation strategies....www.biorxiv.org
@emdatabank.bsky.social EMPIAR entry for stomatin complex from @fravallese.bsky.social and myself released - I hope it will be useful for folks studying #cryoem compositional heterogeneity in pseudo/mixed symmetry systems, as classifying out AQP1 and UT-B was quite a challenge for us at least!
EMPIAR-13732 Stomatin encapsulates aquaporin-1 and urea transporter-B in the erythrocyte membrane EMPIAR, the Electron Microscopy Public Image Archive centered at EMBL-EBI, is a public resource for raw electron microscopy images related to EMDB, contains micrographs, particle sets and tilt-ser...www.ebi.ac.uk
Some progression on 58 kDa GBA as part of my work with @recon4imd.bsky.social. Combined tilted data (Glacios) with careful processing gives a ~4.0 Å map with clear secondary structure features. Still not the resolution that I need for variant studies but a step in the right direction! #CryoEM