Acta Crystallographica Section D: Structural Biology is a leading structural biology journal published by the IUCr. journals.iucr.org/d
A quantitative framework for assessing the cost and utilization of high-end scientific equipment, with a particular application to cryo-electron microscopes in structural biology #EquipmentCostModelling #QueueingTheory #CryoElectronMicroscopy doi.org/10.1107/S205...
Structural studies provide a better understanding of the residues involved in substrate binding and catalysis in a GH5_5 cellulase from Thermoascus aurantiacus #Transglycosylation #Cellulase #GH55Family doi.org/10.1107/S205...
The first crystal structure of gasdermin D bound to a rationally designed peptide ligand reveals peptide engagement at a previously underexplored exosite #GasderminD #Exosite #CyclicPeptides doi.org/10.1107/S205...
To freeze or not to freeze: insights into enzyme crystal structures at room temperature #UrocanateReductase #RoomTemperatureCrystallography #ActiveSiteAnionInteractions doi.org/10.1107/S205...
A comprehensive overview of how X-ray crystallography, cryo-electron microscopy, micro-electron diffraction and cryo-electron tomography have advanced drug discovery across diverse therapeutic areas is provided #XRayCrystallography #CryoEM #CryoET doi.org/10.1107/S205...
Reanalysis of the XFEL data provide structural evidence that supports the assignment of O6 as a state-specific ligand in the S3 state of photosystem II #KokCycle #PhotosystemII #OxygenEvolution doi.org/10.1107/S205...
The crystal structure of Mycobacterium smegmatis MmpL3 in complex with the inhibitor UPAR-1109 at 2.15 Å resolution provides unprecedented insights into the binding mode of the inhibitor #MmpL3 #Indolecarboxamides #CrystalStructure doi.org/10.1107/S205...
Two methods are described for visualizing the H atoms in cryoEM maps of macromolecules #ShoulderPeakDecomposition #RightLeftDifferenceProfilePlot #ModelIndependentMapDecomposition doi.org/10.1107/S205...
The effects of different linkers in fusions of the protein crystallization chaperone 1TEL with target proteins on crystallization were investigated #TELSAM #DARPin #ETV6 doi.org/10.1107/S205...
X-ray-induced spectral changes in common crystallization chemicals and mixtures at cryogenic temperature are mapped: spectroscopic changes can be detected at doses as low as 1 kGy #UVVisAbsorptionSpectroscopy #SpecificRadiationDamage #Radiolysis doi.org/10.1107/S205...
The radiolysis of 5-PMUA exhibits a kinetic transition from flux-limited zero-order decay at RT to retarded target-limited first-order decay at 100 K #RadiationDamage #Enzymology #ReactionKinetics doi.org/10.1107/S205...
A radiation-damage study of the protein rubredoxin from Pyrococcus abyssi at resolutions ranging from 0.54 to 0.57 Å is reported @ActaCrystD @IUCr #RadiationDamage #SubAngstromResolution #FeS4ClusterBondLengths doi.org/10.1107/S205...
The connection between beam damage and image resolution in X-ray and electron microscopy is investigated and the characteristics of electron and X-ray radiolysis are compared to obtain insight into the damage process #RadiationDamage #DoseLimitedResolution #Radiolysis doi.org/10.1107/S205...
D52K, S262Y and D52K/S262Y variants of E. coli flavodiiron-type nitric oxide reductase were introduced in a construct lacking the C-terminal rubredoxin domain #FDP #DiIron #Crystallography doi.org/10.1107/S205...
July issue now available: journals.iucr.org/d/issues/202... The cover highlights Scotty, a tool within the Phasertng codebase that leverages Niggli-cell matches to identify similar crystallographic lattices and perform phasing with difference Fourier methods. Read more at doi.org/10.1107/S205...
A comparison between the use of electrons and X-rays for collecting diffraction data from small protein crystals and imaging data from cells and tissues #XRayCrystallography #ElectronCrystallography #ElectronImaging doi.org/10.1107/S205...
Modulating redox states in heme peroxidases in synchrotron and XFEL experiments #HemeEnzymes #TimeResolvedCrystallography #Peroxidases doi.org/10.1107/S205...
The Alphafuser protein complex prediction pipeline combines experimental interaction data with systematic querying of possible combinations of protein partners in a computationally parsimonious manner @unistra #AlphaFold #Complexes #Multimers doi.org/10.1107/S205...
MiCSPARC leverages automated particle picking and fast 3D refinement times in CryoSPARC to determine the structures of both decorated and undecorated microtubules#CryoEM #Microtubules #HelicalReconstructions doi.org/10.1107/S205...
When the wrong protein wins: hidden contaminants in cryo-EM #CryoElectronMicroscopy #ProteinPurification #EColiContaminants doi.org/10.1107/S205...