Профиль

Initial set of our new T1 T-DNA vectors now available on @addgene.bsky.social: pT1 (no selection marker): lnkd.in/dcnTN8Rj pT1-B (BASTA selection marker): lnkd.in/dgTZmuCx pT1-K (kan selection marker): lnkd.in/dqgv4QRc pT1-H (hygro selection marker): lnkd.in/dhrs5Tsg

Very excited to announce that my paper with @toettch.bsky.social from my time as a Fellow at Princeton's Center for the Physics of Biological Function @cpbf.bsky.social is finally out as a preprint! Here is the link if you want to check it out: www.biorxiv.org/content/10.6... A thread: (1/10)

A modular live-cell biosensor for extracellular protease activityExtracellular protease activity plays key roles in cell and tissue behavior, processing cell surface proteins, ligands and the extracellular matrix. Extracellular proteases can be subject to complex p...www.biorxiv.org

A minimal lipid kinase/phosphatase system, two effector proteins from Legionella pneumophila, self-organizes together with its lipid substrates (PI/PI3P) into dynamic patterns on membranes. Check out our preprint: www.biorxiv.org/content/10.6...

Видео

New preprint! How does a cell "know" how big to make its nucleus? This has puzzled biologists since the 1800’s. We show the answer is physics: colloid osmotic pressure. 🧵1/5

A bit late but…Tenured! Immense gratitude for those who got us here. Firstly the students/postdocs (too many to list!) who took a chance on an unproven lab and were unafraid to pursue some strange new ideas. I couldn't have predicted our path, and I couldn’t be more excited for what's next.

Had a lot of fun writing this dispatch article for @currentbiology.bsky.social with PhD student Caitlin Doyle www.sciencedirect.com/science/arti..., highlighting cell morphogenesis and recent work on suctorian growth control principles. 1/3

We developed a new approach for mapping absolute membrane voltage, based on dynamic photocycle control in Voltron2. www.biorxiv.org/content/10.6... Here's my favorite example: human iPSC colonies break into electrical domains as they begin to differentiate.

The STRAIGHT-IN (Dual) kit is now available via @addgene.bsky.social! 🧬 A complete 24-plasmid toolkit for installing up to two orthogonal CLYBL landing pads, DASIT-based enrichment without FACS, and efficient large DNA integration in human cells. Link: www.addgene.org/kits/gallowa... 🧵

🔬 #JobAlert Interested in doing a PhD with us in #SyntheticBiology on the Reconstitution of Oscillatory Microtubule-Motor Systems? Check shorturl.at/wis5P (note the eligibility criteria) or/and get in touch with us shorturl.at/WmgOD. Deadline: 31 July 2026 #WeAreHiring #AcademicJobs #ScienceJobs

How did octopuses evolve novel traits? Rishav Mitra, Richard Han, Trey Scott, et al serendipitously discover a unique ribosome adaptation that increases the accuracy of protein synthesis and evolved alongside their elaborate nervous systems vs deep sea relatives. www.biorxiv.org/content/10.6...

Congrats to our talented postdoc @amirbitran.bsky.social for his first preprint in the lab, a collaboration with the Bustamante lab, studying time-resolved protein folding during translation elongation with HDX-MS! Check out the attached manuscript and tutorial for more:

Engineering Modular Cargo Loading Strategies for Carboxysome-Derived Protein Particles | ACS Synthetic Biology @cellforganized.bsky.social

Engineering Modular Cargo Loading Strategies for Carboxysome-Derived Protein ParticlesBacterial microcompartments (BMCs) are a diverse and widespread class of self-assembling protein-based organelles consisting of a semipermeable protein shell encapsulating an enzymatic core. Isolated ...pubs.acs.org

Beautiful work (and beautiful figures!) from Sashital lab alum @yuktidhingra.bsky.social! Congrats, and looking forward to seeing more exciting RNAP work in the future!

A High-Affinity Nanobody Recognizing mNeonGreen Enables Versatile Biochemical, Cellular, and in vivo Applications. by Steffen Frey and team: www.biorxiv.org/content/10.6...

Happy Canada Day to all my fellow Canadians out there! 🇨🇦🍁

Our evil ORFeome paper is out! In collaboration with @alex-stark.bsky.social, we screened ~4,000 viral proteins and secreted effectors from bacteria and parasites, covering hundreds of diverse pathogens, for phenotypes in human cells.

Folks, mind the suctorian trap!

I had a ton of fun the other day talking with @nonresidentdesi.bsky.social and @scifri.bsky.social 's Flora Lichtman and Rasha Aridi about murderous microbes and the things that we are trying to learn from them. Happy for the opportunity to bring some new awareness to the world of protists!

Researchers like Ben Larson and Glen D’Souza are piecing together the world of killer microbes, including why they turn on each other and if their tiny weapons could be harnessed to fight disease. Listen to the podcast now: www.sciencefriday.com/segments/kil...

Swords, cannibalism, poison: inside the world of killer microbesSome microbe species have evolved to stab, bomb, cannibalize, or poison each other. Can we harness their weapons for good?www.sciencefriday.com

Tiny unicellular #eukaryotes, big #biomaterial inspiration. Excited to share our Nature Communications study showing that tintinnid ciliates build their chemical- and heat-resistant shells from self-assembling proteins we call Tintinnidorins. doi.org/10.1038/s414... #protistsonsky 1/3