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The STRAIGHT-IN (Dual) kit is now available via @addgene.bsky.social! 🧬 A complete 24-plasmid toolkit for installing up to two orthogonal CLYBL landing pads, DASIT-based enrichment without FACS, and efficient large DNA integration in human cells. Link: www.addgene.org/kits/gallowa... 🧵

⭕️ The STRAIGHT-IN dual plasmids are now available on Addgene! 🧬 @addgene.bsky.social A full STRAIGHT-IN kit with 20+ plasmids, including everything needed to set up and run the platform, is coming soon 🔥 Stay tuned 🚀

Post 1/3 🧵 Our latest work is out at @natbiomedeng.nature.com!🚀 We present STRAIGHT-IN Dual — a platform for precise, allele-specific integration of two large DNA payloads into human iPSCs, enabling more sophisticated control of complex genetic programs. Link📝: www.nature.com/articles/s41... 👇

With Christmas just around the corner, it’s time for the #FluorescenceFriday post🎄! This week, we’re featuring a hiPSC reporter line engineered to express 2 fluorescent proteins—located in the nuclei and actin filaments of #hiPSCs, generated using STRAIGHT-IN Dual🧪🔬 @benjaminbjohnson.bsky.social?

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Here is my 2nd #FluorescenceFriday post! 🧪 #hiPSCs expressing jRCaMP1b, a genetically-encoded Ca2+ indicator 👻. This reporter allows us to record intracellular Ca2+ in hiPSC-derived #cardiomyocytes and cardioids (videos in the comments) #microscopy 🔬 #Science #Biology @benjaminbjohnson.bsky.social

My colleague @benjaminbjohnson.bsky.social has just created a starter pack for hPSC disease modeling. Check it out! 🧪🧫

Hey #BlueSky! Here is my first #FluorescenceFriday post! This cardiac organoid was derived from #hiPSC and imaged using light sheet #microscopy 🔬. 🔵 Cardiomyocytes 🔴 Endothelial cells 🟡 Fibroblasts More coming next week! 🧪 #Science #Biology #CellBiology #SciArt @benjaminbjohnson.bsky.social

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Still a few spots left in the Mammalian Synthetic Biology starter pack!🧪

Please share! Here is a starter pack dedicated to Mammalian SynBio! It is still incomplete so if you would like to be added/removed please let me know! go.bsky.app/SsxHKoV