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1/5: Our latest preprint is out! We show that LEA proteins can diversify particle orientations in cryo-EM, helping to overcome preferred orientation at the air-water interface (AWI). www.biorxiv.org/cgi/content/... with @cijilim.bsky.social and Tim Grant #CryoEM #LEAproteins

Diversifying particle-orientation distributions in cryo-EM with LEA protein additivesPreferential orientation at the air-water interface (AWI) remains a persistent challenge in cryo-electron microscopy, often requiring extensive optimization or specialized grid preparation strategies....www.biorxiv.org

I'm excited to announce a new preprint from the lab just posted to bioRxiv: “Retrograde trafficking inhibitors allosterically trap Get3 to block tail-anchored protein biogenesis.” www.biorxiv.org/content/10.6...

1/11 New preprint from the Sternberg lab in collaboration with the Fernández lab! We are excited to share our structure-function study of DRT10, a bacterial defense-associated reverse transcriptase that synthesizes long tandem-repeat DNA.🧵 Read the full story here: www.biorxiv.org/content/10.6...

Mechanism of tandem-repeat DNA synthesis by an antiviral reverse transcriptaseDefense-associated reverse transcriptases (DRTs) employ DNA synthesis to protect bacteria against phage infection[1][1],[2][2]. We previously showed that DRT10, a tripartite system comprising an RT, a...www.biorxiv.org

Wow, this looks awesome and will be a great resource to anyone studying protein-protein interactions, SLiMs, and cell signaling! Excited to dig into this. Congrats to everyone involved!

Membrane protein solubilization and structure determination using de novo–designed proteins | Science www.science.org/doi/10.1126/...

Membrane protein solubilization and structure determination using de novo–designed proteinsDeveloping therapies and vaccines against integral membrane proteins is hindered by their extensive hydrophobic surfaces, which complicate production and structural analysis. Here, we describe a gener...www.science.org

Meet SpudCell, a synthetic cell made from lifeless ingredients that feeds, grows, divides, and experiences selection. If it doesn't have all the hallmarks of life, it has a lot! Here's my story. Gift link: nyti.ms/4vCbTih

Incredibly proud of Messi Haile @mehsehret.bsky.social, a founding member of the Ho Lab, for her beautiful work uncovering the inner workings of a central piece of the malaria parasite invasion machinery: the malarial moving junction, out today in @cellpress.bsky.social! tinyurl.com/4p3md2eb 🦠❄️🔬

We solved a high resolution structure of the nucleosome core particle directly from nuclear extract from a single T25 flask (3 mL culture). No affinity purification. No sucrose gradients. Just lysate on grids. Raw data available now for anyone who wants to reprocess. www.biorxiv.org/content/10.6...

Quinone-transporting filaments extend the respiratory chain of Gram positive bacteria www.biorxiv.org/content/10.64898/2026.06.26.734721v1 #cryoEM

Wow. I love papers like this where you start off thinking “what? Sounds fishy” and then the data are so convincing and the paper so well written that is all so clear that you are blown away. Cells have a little aerial to allow them to move toward the anode!!! Galvanin! #PureScience #biophysics

Thanks, @olibclarke.bsky.social! Identifying this complex is good news for our job security as experimentalists... As of today, we still can’t predict the full assembly with AlphaFold. So here is an #open_challenge: if anyone can recover the whole complex computationally, I’d love to hear from them!

Nature research paper: Zero-shot design of drug-binding proteins via neural iterative selection−expansion go.nature.com/4eZ6yeW

Zero-shot design of drug-binding proteins via neural iterative selection−expansion - Nature By pairing two neural networks in an iterative optimization algorithm, small-molecule binding proteins can be designed from scratch with high accuracy, affinity and success rates, showing promise for applications in drug delivery and sequestration.go.nature.com

OK— so @phaips.vd.st & @florentwaltz.bsky.social have been testing extensively, and it's pretty clear that MissAlignment may be the best thing to happen to #TeamTomo in the last couple years. One issue: we have to re-process all our data again, hopefully for the last time 😅. Congrats and THANK YOU!!

Announcing BoltzMol-1, BoltzProt-1, and the Boltz API boltz.bio/boltzmol-bol...

Announcing BoltzMol-1, BoltzProt-1, and the Boltz APITwo next-generation open models for small-molecule and protein prediction, now self-serve through the Boltz API.boltz.bio

How did animal senses evolve? Max Coyle et al. used choanoflagellates to uncover TRPW, ancient ion channels predating animal somatosensory receptors. TRPW links microbial prey ecology to evolution of receptor structure, sensory organelles, & multicellular processing. www.biorxiv.org/content/10.6...

Excited to be presenting this work today at the 3DEM Gordon Research Seminar and later this week at the Gordon Research Conference. Looking forward to the discussions and feedback! www.grc.org/three-dimens...

Very sad to learn that Steve White has passed away.

I learned so much from watching past editions of @ccpem.bsky.social Spring Symposium, so I agreed to my talk being recorded even though this is stepping well out of my comfort zone (public speaking isn't my strongest skill). Anyway, here is the recording: www.youtube.com/watch?v=AKTf... #CCPEM

Atomic resolution structure of spinach rubisco reveals protons and dynamics | Guillaume GaullierYouTube video by CCP-EMyoutube.com

In a series of 3 papers and preprints, we’re thrilled to share with you the working laser phase plate. In collaboration with research led by Holger Müller at UC Berkeley, this is a huge innovation in imaging to make small and faint objects inside cells visible. bit.ly/4vK9LVn

Posted this in LinkedIn. Shameless advert for some new structures 👉 lnkd.in/gT9Be3tH. But more structural biologists here! Nice to have Claude control my ChimeraX. At least for visualizations and analysis I can see it really speeding up things. IF you know what you’re doing and what to ask for.

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