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I am very proud to my student Stasia whose manuscript about the nucleocapsid assembly of an ephemerovirus is now available to read. Within three years she managed to defend her thesis, submit the manuscript and secure a postdoc job, while adapting to the UK weather! www.biorxiv.org/content/10.6...

Cryo-electron microscopy structure of the bovine ephemeral fever virus RNA-nucleoprotein assemblyBovine ephemeral fever virus (BEFV), a member of the Rhabdoviridae family, is an arthropod-borne pathogen that causes acute febrile disease in cattle. The structural basis of its genome encapsidation ...www.biorxiv.org

A little summary of some events. Version 1.

@joachimgoedhart.bsky.social & #microscopy friends, we're using ymScarlet-i3. It's the best general purpose red FP we've tried in budding yeast. But in our hands it photobleaches really fast when exposed relatively low-intensity *488 nm* laser light (~20 W/cm^2). Have you seen this? Thoughts?

mScarlet-I3 at FPbasemScarlet-I3 is a basic (constitutively fluorescent) red fluorescent protein published in 2023, derived from synthetic construct. It is reported to be a very rapidly-maturing monomer with low acid sens...www.fpbase.org

This ‘landmark’ study describes the structural mechanism of strand exchange by the RAD51 filament using cryogenic structural, biochemical, and single-molecule analyses. buff.ly/Ieh84gj

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Over five years of work has gone into our new preprint! The #cryoEM structure of a human RAD51 filament caught at the point of strand exchange 🧬 www.biorxiv.org/content/10.1...

Structural mechanism of strand exchange by the RAD51 filament.Homologous Recombination (HR) preserves genomic stability by repairing double-strand DNA breaks and ensuring efficient DNA replication. Central to HR is the strand-exchange reaction taking place within the three-stranded synapsis wherein a RAD51 nucleoprotein filament binds to a donor DNA. Here we present the cryoEM structure of a displacement loop of human RAD51 that captures the synaptic state when the filament has become tightly bound to the donor DNA. The structure elucidates the mechanism of strand exchange by RAD51, including the filament engagement with the donor DNA, the strand invasion and pairing with the complementary sequence of the donor DNA, the capture of the non-complementary strand and the polarity of the strand-exchange reaction. Our findings provide fundamental mechanistic insights into the biochemical reaction of eukaryotic HR. ### Competing Interest Statement The authors have declared no competing interest.www.biorxiv.org

Join the first Keystone conference dedicated to DNA replication gaps and cancer! Send an abstract to be selected for a talk. www.keystonesymposia.org/conferences/...