Профиль

Thank you Alejandro!

Thank you Margarida!

Thanks Max!

Thanks to all coauthors: Julen Mendieta, Ewa Księżopolska, Jim Renema, our beloved proteomics experts Cristina Chiva and Eduard Sabidó @crg.eu, and David Lara, with whom this whole iChIPing adventure of weird species began back when we were young and full of optimism at @weizmann.ac.il

Final thoughts: this work was possible thanks to the protistologists who discovered and cultured these species. Many key cultures are now lost or accumulating mutations after decades growing. Sustained efforts will be needed to reverse this and enable future evolutionary cell biology studies.

The next challenge is to move from chromatin states to their effectors: identifying the proteins that read, implement and connect these states to reveal how changes in chromatin factors shaped the evolution of genome regulation. @seanamontgomery.bsky.social is on it!

H3K79 methylation is the most peculiar case: in some species associated with active transcription, whereas in others linked to repression. In Acanthamoeba even K79me1/2/3 have different associations, possibly explained by the evolution of different Dot1 paralogs.

In contrast, repressive chromatin states varied substantially. Different lineages used distinct combinations of H3K9, H3K27 and H3K79 methylations to mark silent genes and TEs. This may reflect the long evolutionary history of conflicts between eukaryotic genomes and parasitic genetic elements.

What we find? Active chromatin states are remarkably conserved, H3K4me and acetylations mark active promoters, while H3K36me3 tracks active gene bodies. The chromatin architecture of transcription runs deep in eukaryotic evolution.

Now we ask if these conserved marks have a conserved functional readout. We developed a multiplexed ChIP-seq method (iChIP2) to profile 12 marks across 12 species, including some of our favorite protists. In some cases, these represent the first chromatin landscapes for entire eukaryotic lineages.

A few years ago we showed that many histone post-translational modifications and the enzymes that deposit or remove them are ancient and broadly conserved across eukaryotes. www.nature.com/articles/s41...

The previous two: Cell type diversity in Stylophora www.cell.com/cell/fulltex... Facultative symbiosis in Oculina www.nature.com/articles/s41...

Beautiful work! Congrats @chc-evobio.bsky.social, Fred and team.

Congrats @matosches.bsky.social!

Bravo Yehu!

Asymptotic effort

Aggregation in Ministeria induced by bacteria, plus increased mating inside the aggregates. This is really cool! Congrats @multicellgenome.bsky.social and team.

Timely visit for a celebration indeed! I wonder how many EMBO Gold Medals have suze&tonic always ready in their offices...

Huge congrats @dudinlab.bsky.social and @gautamdey.bsky.social. An inspiring example of how collaboration can drive great science. Evolutionary cell biology is on fire! 🔥