One goal of "understanding-by-building" is to test the sufficiency of established models to generate predicted phenotypes in the messy context of living cells. In my postdoc, I asked whether simple two-component circuits could generate self-organised patterns in mammalian cells. 1/4
Supplemental Movie 1Timelapse microscopy of L929 mouse fibroblasts engineered with a synthetic reaction-diffusion circuit, composed of synNotch-mediated juxtacrine activation and paracrine inhibition (JAPI), showing the ...vimeo.com