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Bloom lab

We first measured ability of two H5 HAs to enter cells via sialic acid or MHC-II. As shown below, both HAs could use tufted duck & to lesser extent human MHC-II. [Note: experiments used pseudoviruses, which can only undergo single round of cell entry, providing safe way to study HA]

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Bloom lab

We tested 80 H5 HAs: most but not all could enter cells via tufted duck & to lesser extent human MHC-II. Note MHC-II highly variable within and between species. See dms-vep.org/Flu-H5N1-Ame... for interactive version of below plot.

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Ответы

Bloom lab

To understand how HA interacts w MHC-II, we measured how all H5 HA mutations affect pseudovirus entry via sialic acid or tufted duck MHC-II. Identified loss-of-function mutants that could only use MHC-II or sialic acid.

Ответ для Bloom lab
Bloom lab

The mutations that reduced MHC-II entry clustered in a region on HA head defining the MHC-II binding surface. We also directly measured how HA mutations affect binding to tufted duck MHC-II, and identified same binding surface.