Preprint alert❗ STRAIGHT-IN Dual, a platform for dual, single-copy integrations of DNA payloads and gene circuits into human induced pluripotent stem cells. This platform allows the generation of genetically-modified hiPSC lines in 1 week with 100% efficiency! doi.org/10.1101/2024...
STRAIGHT-IN Dual: a platform for dual, single-copy integrations of DNA payloads and gene circuits into human induced pluripotent stem cellsTargeting DNA payloads into human (h)iPSCs involves multiple time-consuming, inefficient steps that must be repeated for each construct. Here, we present STRAIGHT-IN Dual, which enables simultaneous, ...doi.org