Structural basis of biofilm formation mediated by the Pseudomonas aeruginosa fibrillar adhesin CdrA led by @olivia--smith.bsky.social Collaboration with @alexbateman1.bsky.social , Andres Floto and @geiselbiofilm.bsky.social labs
Профиль
Tanmay Bharat
Профиль VivelyMRC-LMB #cryoET #biofilms #S-layers #antibiotics https://www2.mrc-lmb.cam.ac.uk/groups/bharat/
Disrupting phage liquid crystalline droplets restores antibiotic susceptibility in Pseudomonas aeruginosa biofilms out in @plosbiology.org by @abultarafder.bsky.social and team. Exciting collaboration with @geiselbiofilm.bsky.social @pearce-maths.bsky.social and others
PLOS Biology#Biofilm matrices containing filamentous phages help #Pseudomonas aeruginosa tolerate antibiotics. @abultarafder.bsky.social @tbharat-lab.bsky.social &co show that #nanobody disruption of #phage Pf4 #LiquidCrystalline droplets restores #antibiotic susceptibility @plosbiology.org 🧪 plos.io/4xkd6Mw
Sub-cellular chemical mapping using correlated cryogenic electron and mass spectrometry imaging online @natmethods.nature.com, led by Hannah Ochner, collaboration with @catfranco.bsky.social and @kiranrpatil.bsky.social labs doi.org/10.1038/s415...
Applications and prospects of cryo-electron tomography in drug discovery and understanding disease Review article by @camilaclemente.bsky.social in our lab www.sciencedirect.com/science/arti...
Applications and prospects of cryo-electron tomography in drug discovery and understanding diseaseCryo-electron tomography (cryo-ET) is emerging as a transformative tool for structural biology. Unlike methods based on purified molecules, cryo-ET en…www.sciencedirect.comInteraction of Pf4 tactoids with bacteria and synthetic colloidal rods Important biophysical measurements on the Pseudomonas aeruginosa biofilm-related phage Pf4 by Dirk Aarts laboratory with @abultarafder.bsky.social link.springer.com/article/10.1...
Interaction of Pf4 tactoids with bacteria and synthetic colloidal rods - European Biophysics JournalWe investigated the interaction of liquid crystal droplets, formed by a phase separating system of elongated virus particles and polymer with rod-like impurities. The virus particles were Pf4, which w...link.springer.comOxygen gradients reshape cross-feeding through emergent spatial organization of gut commensal bacteria www.biorxiv.org/content/10.6... Use of isotope labels and cryo-CLEM-FIB-SIMS to study microbial communities by Hannah Ochner. Collaboration with @kiranrpatil.bsky.social @jmghigolab.bsky.social
Oxygen gradients reshape cross-feeding through emergent spatial organization of gut commensal bacteriaMicrobial interactions unfold within environments structured by physical transport and chemical gradients. Yet most mechanistic studies rely on well-mixed systems that mask the reciprocal influences of environmental heterogeneity on metabolism and ecology. Here, we investigate how the physical environment modulates the interaction between the gut commensal Bacteroides thetaiotaomicron and Escherichia coli . In anoxic liquid culture, cell-resolved isotope imaging and genetic perturbations reveal exploitative cross-feeding, where E. coli consumes diffusible sugars released by B . thetaiotaomicron during starch degradation. When exposed to intestinal-like oxygen gradients in microfluidics, the interaction is restructured by spatial organization. The species self-organize into complementary niches: E. coli locally depletes sugars and oxygen, thereby expanding the anoxic niche required by B. thetaiotaomicron . A reactive transport model confirms that this organization arises from coupled feedback between physical transport and metabolic reaction rates. Together, our results reveal how physical structure and chemical gradients convert an exploitative cross-feeding interaction into a dynamic niche-construction process that generates emergent spatial organization and stabilizes coexistence. ### Competing Interest Statement The authors have declared no competing interest.www.biorxiv.orgAssembly, architecture and functional roles of microbial surface layers Review article published in @natrevmicro.nature.com with @bupbuse.bsky.social, Andriko von Kügelgen and @vikramalva.bsky.social. S-layers are everywhere! www.nature.com/articles/s41...
Assembly, architecture and functional roles of microbial surface layers - Nature Reviews MicrobiologySurface layers (S-layers) are ubiquitous protein assemblies that coat prokaryotic cells, with their functional roles increasingly coming into focus. In this Review, Isbilir and colleagues discuss rece...www.nature.comAn Asgard archaeon with internal membrane compartments Brilliant study led by @fmacleod.bsky.social and Andriko von Kügelgen. Tight collaboration with @buzzbaum.bsky.social and lab. Congrats to all authors! www.biorxiv.org/content/10.1...