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And the cruise on the Seine.

Impressive bench to bedside, Agnete Kirkeby, promise of pluripotent cell technologies. To end our 2-week course, a dinner cruise on the Seine. Magical evening, great company, students and instructors made this a successful and fruitful exchange of ideas. Thanks to all! See you in 2027 #ASCB course!

Matthias Lütolf engineered his way through organ regeneration with impressive micropatterns/microfluidics/imaging - to recapitulate growth and oncogenesis.

A travel through development and tumour biology with Cédric Blanpain, the power of lineage tracing, modelling and imaging! Then chick and quail: Charlène Guillot with Olinda Alegria-Prevot - ex ovo electroporation of embryos - viewing segmentation/growth, beautiful to watch!

The importance of history, clarity on what we generate in a dish compared to the ground truth. The resourceful David Turner @gastruloids.bsky.social, initiated students on reproducible gastruloid generation and A-P extension. And now, into the 2nd week...

After our Friday night picnic + sunset with Eiffel on the rooftop, the week ended with an eye-opening perspective lecture by @amartinezarias.bsky.social on mouse, human, multispecies embryo models; from bench to birth.

Thanks also to Stefan Rulands for the didactic and contextual lecture integrating theory and practice on the use and limitations of models!

Intestinal organoids with Giulia Nigro and Aline Stedman - following a wonderful presentation once again by Marc Van de Wetering - from history to present day “gut” and other types of “organisated-oids”. The promise, practise, and limitations. Many thanks for the guidance!

A swim into the zebrafish brain with powerful genetic tracing of neural stem cells @zenlabpasteur.bsky.social, Laure Bally-Cuif, Sara Ortica, Tanya Foley, Isabelle Foucher. Beautiful demonstration of the power of a model organism! Thanks to all!

Laura Cantini & Stephan Fisher gave excellent lectures on challenges with omics, QC and data interpretation, and how to navigate emerging technologies. Thank you for the clarity!

First dynamic in vivo analysis of dystrophic MuSCs. Identifies niche dysfunctions and points to strategies to restore stem cell function in DMD.

Cross-grafting experiments uncouple MuSC behaviours: Migration is dictated by the myofibre niche, fate decisions are fibre-independent, and symmetric cell divisions are disrupted. Resolves some long-standing debates in the field.

mdx stem cell dysfunction is driven by p38/PI3K signalling, unlike healthy MuSCs, leading to precocious differentiation and loss of stemness.

and picnic-ing...

Relaxing on the rooftop, an evening picnic with students and instructors

Taking us through axial elongation is David Turner @gastruloids.bsky.social, a great teacher of ES cell handling and formulating protocols for reproducible gastruloid generation. A-P extension, and into the 2nd week.

Superb dual lectures by @amartinezarias.bsky.social on mouse, human, multispecies, embryology, gastruloids; from bench to birth - discussions on the dynamic nature of the field. Importance of history, context, and remaining lucid while discovering. Much to reflect upon, as we explore.

Isolation of small intestine and colon crypts to make organoids with Giulia Nigro, Aline Stedman, Sophie Dulauroy - following a great lecture by Marc Van de Wetering into the history and organoid biology of the gut. Great exercise on how to handle samples with the utmost care.

Isolation of muscle fibres and their stem (satellite) cells. Plate and wait, from quiescence to differentiation - following Shahragim’s lecture on muscle stem cell emergence and diversity. @tajbakhshlab.bsky.social, @brendan-evano.bsky.social, Barbara Gayraud-Morel. Patience is a virtue.

Laura Cantini & Stephan Fisher lectures guided us through the trials and tribulations of omics - the power and drawbacks of data integration, and how to navigate the minefield. Thanks!