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In E. coli, PepA is a multifunctional enzyme involved in protein degradation, plasmid segregation, gene regulation. In Vibrio cholerae, PepA regulates > 100 genes and is even involved in controlling Na+ homeostasis. Important for an aquatic bacterium! 👉 journals.asm.org/doi/10.1128/... @asm.org

The evolution of a Na+-sensitive Vibrio cholerae mutant unmasks the moonlighting aminopeptidase PepA as a regulator of nhaB Na+/H+ antiporter gene expression | Journal of BacteriologyThe emergence and spread of multi-resistant bacteria are a major problem for humans and animals. Therefore, the identification of novel targets for the development of novel antibiotics to combat patho...journals.asm.org

👉 RNA quality control enables antibiotic tolerance: www.cell.com/cell-host-mi... @cp-cellhostmicrobe.bsky.social #RNAseY

RNA quality control enables antibiotic toleranceNishimoto et al. experimentally evolve bacteria during infection, revealing a ribosomal “crash and reboot” strategy as a mechanism of antibiotic tolerance. Mutations in the RNA degradosome allow RNA t...www.cell.com

👉 The Master Regulator Spo0A Integrates DNA Sequence and Shape to Regulate the Sporulation Kinase Gene kinA in Bacillus subtilis: onlinelibrary.wiley.com/doi/10.1111/... #subtiwiki

The Master Regulator <fc>Spo0A</fc> Integrates <fc>DNA</fc> Sequence and Shape to Regulate the Sporulation Kinase Gene <fi>kinA</fi> in <fi>Bacillus subtilis</fi>During starvation, progressive Spo0A~P accumulation is decoded by three Spo0A~P-binding sites and intrinsic DNA bending within the kinA promoter. Together, these features fine-tune kinA transcription...onlinelibrary.wiley.com

Wow! There are still exciting things to discover in the field of c-di-AMP metabolism: A c-di-AMP receptor mediates epithelial adhesion of Lactiplantibacillus plantarum! www.nature.com/articles/s41... @natcomms.nature.com

In @eLife.bsky.social: Three metabolic pathways replenishing the one-carbon pool collectively support growth and virulence of Listeria monocytogenes! doi.org/10.7554/eLif...

The power of adaptive evolution: Pathway remodeling by spontaneous mutations an selection allows efficient co-utilization of glucose and xylose in Escherichia coli: 👇 | mBio journals.asm.org/doi/10.1128/...

Pathway remodeling and adaptive evolution enable efficient co-utilization of glucose and xylose in Escherichia coli | mBioThe inability of industrial microbes to co-utilize glucose and xylose severely limits lignocellulosic biomass valorization in bioproduction. Here, we combined pathway separation and adaptive laborator...journals.asm.org

Advanced Kaneology

👉 A safeguarding factor monitors septal pore stability to secure compartmentalization during sporulation in Bacillus subtilis: www.nature.com/articles/s42... #subtiwiki

A safeguarding factor monitors septal pore stability to secure compartmentalization during sporulation in Bacillus subtilis - Communications BiologyIdentification of CprV during bacterial sporulation reveals insight into how developing cells monitor compartmental integrity during cellular remodelling and differentiation.www.nature.com

👉 The B. subtilis translesion polymerase Pol Y1 is not strongly recruited to sites of replication upon different types of DNA damage 👇 dx.plos.org/10.1371/jour... #subtiwiki

The B. subtilis translesion polymerase Pol Y1 is not strongly recruited to sites of replication upon different types of DNA damageAuthor summary Unrepaired DNA damage can stall the process of normal DNA replication, ultimately leading to cell death. One pathway for resolving this stall is translesion synthesis (TLS), in which sp...dx.plos.org

👉 phnE frameshift reversion frequency: beyond density effects to genomic prevalence In, The Journal of Bacteriology journals.asm.org/doi/10.1128/... #phosphonates #glyphosate #mutation

phnE frameshift reversion frequency: beyond density effects to genomic prevalence | Journal of BacteriologyPhosphonates are an important phosphorus reservoir in many environments, and their utilization by Escherichia coli depends on the phn operon. This study reexamines the unusually high frequencies of phnE revertants reported for laboratory K-12 strains. By controlling for phosphate contamination in selective media, we show that reversion frequencies are substantially lower than previously estimated and largely independent of cell density. We further find that restoration of phnE function does not confer a detectable fitness advantage under phosphate limitation. Consistent with this observation, genomic analyses reveal that loss of phn function is common among natural E. coli isolates. Together, these findings suggest that loss of phn function may represent an adaptive strategy in a substantial fraction of natural populations.journals.asm.org

😱 RelA-mediated (p)ppGpp accumulation links glucose starvation to peptidoglycan synthesis arrest and spherical morphogenesis via PBP2x and FtsX in Streptococcus suis In, The Journal of Bacteriology journals.asm.org/doi/10.1128/...

RelA-mediated (p)ppGpp accumulation links glucose starvation to peptidoglycan synthesis arrest and spherical morphogenesis via PBP2x and FtsX in Streptococcus suis | Journal of BacteriologyBacterial survival depends on adapting morphogenesis to nutrient availability, yet the molecular links between carbon starvation and cell division remain poorly defined. In the zoonotic pathogen Strep...journals.asm.org

🫴 Trehalose metabolism and its impact on PrfA activity in Listeria monocytogenes In, The Journal of Bacteriology journals.asm.org/doi/10.1128/... #listeria

Trehalose metabolism and its impact on PrfA activity in Listeria monocytogenes | Journal of BacteriologyVirulence factors determine the pathogenic potential of bacteria; however, constant expression of these factors is an energetic burden. In Listeria monocytogenes, virulence gene expression is induced by the virulence regulator PrfA, whose activity is repressed in the presence of readily metabolizable sugars. This study reveals that trehalose, unlike other phosphotransferase system (PTS)-imported sugars, supports listerial growth while barely affecting PrfA activity, highlighting an exception to the established model of sugar-dependent PrfA inhibition. Based on 13C-labeling experiments, we hypothesize that the metabolic flux rather than the sugar type alone influences PrfA activity. The proposed link between phosphoenolpyruvate availability and PrfA activity offers new insight into how nutrient sensing is linked to pathogenicity.journals.asm.org

🫴 From hijackers to architects: the role of prophages in bacterial genome evolution In The Journal of Bacteriology journals.asm.org/doi/10.1128/...

From hijackers to architects: the role of prophages in bacterial genome evolution | Journal of BacteriologyBacteriophages (phages) are one of the most important drivers of bacterial evolution, regulating physiology, genetic diversity, population dynamics, and coevolution through complex host-phage interact...journals.asm.org

👉 Bacteria sense virus-induced genome degradation via methylated mononucleotides: | Science www.science.org/doi/10.1126/...

Bacteria sense virus-induced genome degradation via methylated mononucleotidesPhages often degrade the genome of their bacterial host to individual nucleotides. Here we describe Metis, a bacterial defense system that directly senses phage-mediated host genome degradation. Metis...www.science.org

The world is surely already waiting for the next paper. The good news: the wait will soon be over! This little gem was created through a fantastic collaboration! @asm.org

🫴 The genome sequence of Bacillus anthracis strain “Bundesheer” isolated from a postal sack in the US embassy Vienna in 2001 during the Amerithrax incident in the US | Microbiology Resource Announcements journals.asm.org/doi/10.1128/... #bundesheer

The genome sequence of Bacillus anthracis strain “Bundesheer” isolated from a postal sack in the US embassy Vienna in 2001 during the Amerithrax incident in the US | Microbiology Resource Announcement...The Gram-positive spore-former Bacillus anthracis played the central role in the 2001 Amerithrax letter attacks in the United States (1). During that time, the Austrian Armed Forces were conducting an...journals.asm.org

👉 Sustained ppGpp production underpins months-long survival of a bacterium in growth arrest: | mBio journals.asm.org/doi/10.1128/...

Sustained ppGpp production underpins months-long survival of a bacterium in growth arrest | mBioThe molecular basis for long-term survival of starved bacteria is not well understood. The bacterium Rhodopseudomonas palustris has a metabolism in which it survives in growth arrest for months, as lo...journals.asm.org

During this year’s graduation ceremony, the bachelor thesis of Mr. Florian Roth was awarded with the Faculty Prize. Mr. Roth contributed to a better understanding of the role of a multifunctional aminopeptidase in sodium ion homeostasis in the human-pathogenic bacterium Vibrio cholerae!

👉 Dynamic assembly of malate dehydrogenase–citrate synthase multienzyme complex in the mitochondria: doi.org/10.7554/eLif...

Dynamic assembly of malate dehydrogenase–citrate synthase multienzyme complex in the mitochondriaThe yeast mitochondrial malate dehydrogenase–citrate synthase metabolon is a dynamic multienzyme complex that continuously assembles and disassembles in response to respiratory activity, mitochondrial...doi.org

🤔 I was not aware that a glyoxylate shunt exists in Bacillus subtilis....(?)! Guckstdu hier: www.sciencedirect.com/science/arti... #subtiwiki