Профиль

if you want to read our full story on alarmone and plant immunity: bsky.app/profile/fede...

Finally, but not least, i'd like to thank @nicocecchini.bsky.social and @benfield1.bsky.social that greatly contributed to my PhD! Moreover, this work wouldn't have been possible without the rest of the authors: Iván, Flor, Pedro, Rocío, Cami, Denisse, Carlos, etc!

This paper and my PhD would not be possible at all without the support of my (astonishing) supervisor Ezequiel Petrillo (aka petry) 🤞🏽.

If you want to find out more about pathogen, alarmone, regulation of stomatal aperture and photosynthesis efficiency and Botrytis-induced effects, read the full story below 👇 www.biorxiv.org/content/10.6...

Alarmone ((p)ppGpp) signalling tunes Arabidopsis thaliana nuclear gene expression to shape the balance of plant immune outcomesRSH enzymes (RelA/SpoT homologs) synthesize ppGpp (guanosine tetra-/pentaphosphate) in plastids, regulating organelle function. More importantly, whether ppGpp acts as a simple rheostat on immune outp...www.biorxiv.org

What about necrotrophic pathogens? Well, ppGpp is required for the resistance against these pathogens (i.e: Botrytis cinerea)!!!!

Also, we found that ppGpp controls photosynthetic efficiency during pathogen infection. Moreover, we demonstrated that pathogen T3SS is required for ppGpp-mediated downregulation of photosynthetic efficiency.

so, we decided to carry on and infect (with Pseudomonas) plants with high and null levels of ppGpp. High levels of ppGpp make the plants more sensitive to this pathogen while null plants are more resistant. We confirmed this by using a line with an inducible ppGpp synthase👇

Surprisingly, SA pathway, but not SA itself, is required for the induction of ppGpp biosynthesis genes during pathogen infection.😨

Ok, so ppGpp deregulates immunity-linked genes, but is this molecule produced under infection conditions? By infecting plants with Pseudomonas (hemi-biotrophic) and Botrytis (necrotrophic), we found that ppGpp biosynthesis genes are induced alongside with JA and SA genes.

As every crisis means opportunity time, we did find that plant alarmone induces a deregulation of nuclear-encoded immunity-related genes, in normal conditions!

At the very beginning of my PhD we decided to check whether this molecule can modify the light- and dark-related transcriptomes. Well, guess what, no 😪. The main hypothesis of my PhD had fallen apart at that point.

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