News from Jennifer Doudna's lab at UC Berkeley, Innovative Genomics Institute. Tweets from lab members and not Jennifer Doudna unless signed JD. Tweets represent personal views only. doudnalab.org
First preprint from the lab of postdoc alumni @jnoms.bsky.social. Check it out!
Jason NomburgHappy to share the first preprint out of the Nomburg lab! Many aspects of cellular immunity are shared across the tree of life. Here, we show that some of these conserved aspects of cellular immunity are mirrored by conserved effectors of immune antagonism. Thread below! 1/15
Our AI-guided design of RNA-guided nucleases is now out in @science.org 💻🧬 We used structure and evolutionary data to generate minimal, active and divergent gene editors! www.science.org/doi/10.1126/... Congrats to @petrskopintsev.bsky.social @isabelesain.bsky.social @evandeturk.bsky.social & team!
Structure and evolution-guided design of minimal RNA-guided nucleasesThe design of RNA-guided nucleases with properties not limited by evolution can expand programmable genome-editing capabilities. However, generating diverse multidomain proteins with robust enzymatic ...www.science.orgHuge congratulations to Doudna Lab postdoc, Santiago!
Innovative Genomics InstituteCongratulations to Santiago López in the @doudna-lab.bsky.social at the IGI for being selected for the 2026 class of the Pew Latin American Fellows Program in the Biomedical Sciences! Learn about Santiago's work and the other scholars here: www.pew.org/en/about/new...
We now present our latest work, now out in @nature.com! A creative new CRISPR-based approach can selectively destroy cells carrying undruggable mutations in cancer. Work led by @jingkunzeng.bsky.social In collaboration with Alan Ashworth, Yang Liu and Ryan Jackson. www.nature.com/articles/s41...
Targeting Cancer-Specific Mutations with RNA-Triggered Chromatin Shredding - NatureNature - Targeting Cancer-Specific Mutations with RNA-Triggered Chromatin Shreddingwww.nature.comNew pre-print 📣 “Undruggable” cancer mutations remain very hard to target with current modalities. What if we could instead sense mutant transcripts and convert that recognition into selective cell killing? Check out this work led by postdoc Jingkun Zeng: www.biorxiv.org/content/10.6...
www.biorxiv.orgWe present VIPR, a phage-encoded RNA-guided system that recognizes DNA with a noncontiguous code unlike CRISPR. Tiny, programmable, possibly ancestral to CRISPR immunity; VIPR is built around a striking RNA-DNA-DNA triplex.
Kenneth LoiExcited to share our discovery of a new programmable RNA-guided DNA-targeting system hiding inside bacteriophages that predates CRISPR. We call it VIPR (Viral Interference Programmable Repeat), and it uses an entirely new logic to find its targets. Thread + link below.
🚀 New preprint! @jacobsenucla.bsky.social x @doudna-lab.bsky.social collaboration: High-activity TnpB (Ymu1-WFR) + a multi-gRNA system in TRV enables heritable, tissue-culture-free multiplex editing in plants. Big potential for plant biotech 🧬🌾🌱 doi.org/10.64898/202...
doi.orgNew preprint 👉Doudna x Bryant x Jacobsen x Savage collaboration! Work led by @zehanzhou.bsky.social, I. Saffarian-Deemyad, @honglue.bsky.social, T. Weiss We dissect how stepwise DNA unwinding gates TnpB genome editing, revealing how unwound DNA states enhance cleavage www.biorxiv.org/content/10.6...
Stepwise DNA unwinding gates TnpB genome-editing activityTnpB is a compact RNA-guided endonuclease and evolutionary ancestor of CRISPR-Cas12 that offers a promising platform for genome engineering. However, the genome-editing activity of TnpBs remains limit...www.biorxiv.orgNow online at @cp-cellhostmicrobe.bsky.social from co-first authors @erinedoherty.bsky.social & @jnoms.bsky.social
Erin DohertyWork I co-led with @jnoms.bsky.social is now online at @cp-cellhostmicrobe.bsky.social ! We revealed a previously unrealized diversity of viral immune-evasion proteins that selectively destroy different cyclic nucleotide signals used in bacterial immunity. www.sciencedirect.com/science/arti...