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Fresh cut ✂️

I will forgive the photosynthesis comment bc your compared me to your favorite animal :p

Incredibly grateful for the amazing mentorship I’ve had over the years @cellarchlab.com @edwardmarcotte.bsky.social David Taylor and @jbwallingford.bsky.social and can’t wait to pay it forward :D

I will be hiring at all levels so if you are interested and want to learn more, please get in touch! 📻 ❄️ 🔬 🦠 🧬 #cilia #cryoet #proteomics #exm #protistsonsky

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#cilia #cryoet #proteomics

And over the years several people in the @cellarchlab.com contributed to this work including @phaips.vd.st and @lifeonthewedge.bsky.social

This project was started over 10 yrs ago between @cellarchlab.com and @stearnslab.bsky.social, I think I was taking organic chemistry at the time 😝. @hugovdhoek.bsky.social heroically put in a huge effort to get these cells on grids and acquire some beautiful tilt series 💪

Using this powerful combo of tools we observe other features of the ciliary base including the ciliary necklace aligned w the transition zone, IFT trains present at mature cilia and undocked centrioles near ciliary vesicles, and a cytoskeleton network of bundled actin and intermediate filaments

While XL/MS provided several candidates for microtubule binding proteins, the subciliary localization was further verified by @marinebrunet.bsky.social w U-ExM. This combo identified several new microtubule binding proteins including DNAJB6, which is abundant in the transition zone and axoneme

To supplement our cryo-ET and generate candidates for proteins that localize to different ciliary regions, we took a trip to the local butcher to pick up some cow trachea to build a mammalian multiciliated cell interactome using XL/MS (enjoy this vid of @cellarchlab.com shaking cilia off trachea)

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Using subtomogram averaging and classification, we map out structural changes along the microtubules of the ciliary base, highlighting densities specific to each region. The transition zone is marked by distinct features including the A-B linker, outer helical wrap, MIP helix, and absence of NME7

I’m an optimist 👀

Congrats Helen!! Really cool story

Cool!! Can’t wait to try

😭😭 That is so kind, just continuing to explore all the foundational research that came before!!

Halloween card?!

And the INCREDIBLE biophysics and bioEM facilities and protein production facility (Niko and Roman)

This production was the result of a huge team effort from the members of @cellarchlab.com, Maier lab, Lim lab, Hiller lab and our neighbor Basile Wicky!