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Профиль

Anh Hoang Le, PhD 🏳️‍🌈

Профиль Vively

Sir Henry Wellcome fellow studying macrophage migration and mechanobiology @UCL. BSc Biochemistry @Bristol. PhD Cancer Biology @CRUK Scotland Institute. 🏴󠁧󠁢󠁳󠁣󠁴󠁿 Cell lover by day, takeaway lover by night. https://hoanganhle2602.wixsite.com/cellsandtheirwonders

Happy #FluorescenceFriday. Embryonic myeloid cells are curious. They emerge early during development and disperse throughout an embryo. During this journey, they interact with different cell populations. This myeloid cell 🔴 is seemingly probing ectoderm cells 🔵 to the point of warping their nuclei.

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Yey, I figured out how to display 3D movie stacks in #ChimeraX, thanks to @dabiophysicist.bsky.social for the suggestion. My original tiff was not ome-tiff so you'll need to convert it first (do this with #Python), then adjust voxelsize in ChimeraX so the image isn't flat, record, and voila!

Anh Hoang Le, PhD 🏳️‍🌈

One drawback tho and unless I'm not aware of an alternative, it seems if you want to display a time series stack, you have to save each time frame as a separate stack before loading them into #ChimeraX. If you don't, it will superimpose all timepoints into 1 single 3D stack. This, #napari is better!

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Happy #FluorescenceFriday. Its always exciting when i get to test out new constructs in my cells. It's not common that I get to see the ER network in an immune cell. This construct is not strictly ER but it's so overly expressed that it labels the ER in an embryonic macrophage in 🐸. How cool?

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One drawback tho and unless I'm not aware of an alternative, it seems if you want to display a time series stack, you have to save each time frame as a separate stack before loading them into #ChimeraX. If you don't, it will superimpose all timepoints into 1 single 3D stack. This, #napari is better!

Anh Hoang Le, PhD 🏳️‍🌈

So it turns out that you can visualise 3D cells really well #chimeraX, better than #napari dare I say. Very intuitive. You can adjust different representations, surface, volume, etc, and to make a movie. I'm blown away because I learned ChimeraX for protein structure visualisation, not cells! 🤯

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So it turns out that you can visualise 3D cells really well #chimeraX, better than #napari dare I say. Very intuitive. You can adjust different representations, surface, volume, etc, and to make a movie. I'm blown away because I learned ChimeraX for protein structure visualisation, not cells! 🤯

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I love these PIP3 biosensors because it labels the plasma membrane so well. You can see the cell's edge so clearly along with any intracellular vesicles. Also, this cell is so blebby. This video was taken when I was still working on endocytosis :D Good old times! #FluorescenceFriday

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Anyone know a good 3D viewer plug in/software? I tried 3D viewer on #Fiji, it looks good but there seems to be some bugs that makes manipulating and moving objects around so difficult. If you hold Shift and move, the entire object disappears for example. #question

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Another #FluorescenceFriday. When the world is going crazy, I find some peace watching at these myeloid cells (🔴) going about their day. They exist in tissue surrounded by other cells (🔵), and you can see them popping up and down, going in and out of plane as they explore their surrounding.

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Had my wisdom tooth taken out today (finally). But absolutely traumatised. The dentist was amazing and very efficient, but didnt expect how the procedure is done (thats my bad). Thought it'd be more elegant and cutesy, but no, I thought my jaw was gonna snap in half 😭. Took only 10min tho 👍

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