Happy #FluorescenceFriday. Embryonic myeloid cells are curious. They emerge early during development and disperse throughout an embryo. During this journey, they interact with different cell populations. This myeloid cell 🔴 is seemingly probing ectoderm cells 🔵 to the point of warping their nuclei.
Профиль
Anh Hoang Le, PhD 🏳️🌈
Профиль VivelySir Henry Wellcome fellow studying macrophage migration and mechanobiology @UCL. BSc Biochemistry @Bristol. PhD Cancer Biology @CRUK Scotland Institute. 🏴 Cell lover by day, takeaway lover by night. https://hoanganhle2602.wixsite.com/cellsandtheirwonders
Yey, I figured out how to display 3D movie stacks in #ChimeraX, thanks to @dabiophysicist.bsky.social for the suggestion. My original tiff was not ome-tiff so you'll need to convert it first (do this with #Python), then adjust voxelsize in ChimeraX so the image isn't flat, record, and voila!
Anh Hoang Le, PhD 🏳️🌈One drawback tho and unless I'm not aware of an alternative, it seems if you want to display a time series stack, you have to save each time frame as a separate stack before loading them into #ChimeraX. If you don't, it will superimpose all timepoints into 1 single 3D stack. This, #napari is better!
One drawback tho and unless I'm not aware of an alternative, it seems if you want to display a time series stack, you have to save each time frame as a separate stack before loading them into #ChimeraX. If you don't, it will superimpose all timepoints into 1 single 3D stack. This, #napari is better!
Anh Hoang Le, PhD 🏳️🌈So it turns out that you can visualise 3D cells really well #chimeraX, better than #napari dare I say. Very intuitive. You can adjust different representations, surface, volume, etc, and to make a movie. I'm blown away because I learned ChimeraX for protein structure visualisation, not cells! 🤯