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Why not?!

What’s the new recipe? I shouldn’t change but we are so attracted to novelty…

My biggest recent refinement was the pause before stretching. In a pizzeria they always start from a ball, but the opposite works much better for me. Idea from this great video by Kirsty Cameron, from Ooni’s YouTube. youtu.be/joGKYTGbVw8

How to Make Neapolitan-style Pizza | Making Pizza At HomeYouTube video by Ooniyoutu.be

Great! I’m very jealous. Complete disaster here (northern Italy)- the relatively young tree (2 year old) grew fruits but lost all its leaves in the recent heatwave. The head of the family council thinks it is dead/dying. I’m irrigating daily in the hope of saving it.

The Ooni recipe works well for me, even better with “pizza flour”. But, 36h in advance, in fridge, much less yeast, and allow each ball to relax 1h before. ooni.com/blogs/recipe...

Classic Pizza DoughFlour, salt, water and yeast come together to create the perfect foundation for any good Italian pizza. Say hello to our original pizza dough recipe!ooni.com

Perfect intersection of my childhood and my professional life!

We love Culinary Class Wars. Have not seen season 2 yet. Bonus- you learn a lot about Korean food techniques and cooking philosophy.

Thanks @igreger.bsky.social !

Thanks Sven. I should mention, all physiology work was done with a fantastic custom microscope built for us in about 2014 by @rappopto.bsky.social A real workhorse! This scope (including a xy scanner for 405 nm laser uncaging) has been moved 6 times!!! And still works optimally.

Well done Christophe. No mean feat.

Thank you Mark, i really appreciate it. I think we will still continue to use it…plenty still to investigate!

Thank you, Tom!

Crap, I forgot to thank @markcembrowski.bsky.social NElson Spruston and HHMI Janelia team for the HippoSeq transcriptomic database that basically enabled this entire discovery. Other sources more or less agree but this is the most precise. Fantastic resource for the community! hipposeq.janelia.org

Thanks for checking out HippoSeq. We have put together an example exploratory workflow to analyze differences in gene expression in CA3 pyramidal cells.hipposeq.janelia.org

Thanks to great postdocs Marin Boutonnet, Jenn Noonan and the original slow AMPA Nico Pampaloni for their tireless work chasing this dream. Thanks to the wonderful Viral Core Facility of Charité for their help. And thanks to the DFG, EMBO and NSERC Canada for funding this work. More to come! /end

We don’t yet know all that slow AMPA can do. It might unlock NMDA, maybe synaptic plasticity. It's slowness could be important for cognitive processes. The craphical abstract from 2021 shows key ideas: STP, diversity and detonation. But now we do know: CNIH3 is a main actor for slow AMPA! 20/n

Most important, I think, is that slow AMPA can make any synapse into a super-powered conditional detonator (that is, one connection can spike the target cell). We can have detonators anywhere in the brain….important for network function and topology. 19/n

Slow AMPA is more dangerous than NMDA, being slow and directly activated by glutamate but not damped by Mg2+. ThIs unchecked excitation is probably why it is sparsely expressed. In overexpression, the slice really suffers. 18/n

CNIH3 is not just a marker, it's a great candidate for slow AMPA. Jenn Noonan did tough experiments in HEK cells (everything dies...) to show CNIH3 makes AMPARs very slow, at least 100 ms decay and a big reduction of desensitisation (confirming results from Bowie, Nakagawa and Cull-Candy labs). 17/n

Even more amazing, overexpressing CNIH3 in dorsal CA1 introduced mosaic expression. Some cells resist, and some synapses stay fast. This “silent” diversity of synapses to accept or reject slow AMPA is really fascinating, and suggests synaptic diversity has several levels. 16/n

We were amazed to see that two different shRNAs against CNIH3 (which should spare CNIH2, AMPA nerds) completely removed all trace of slow AMPA. Not reduced - GONE! A scrambled control shRNA did not affect slow AMPA. 15/n