Attention Nicotiana benthamiana users! Certain strains of Agrobacterium (e.g. GV3101-pMP90) induce glandular trichomes because of cytokinin production by Agrobacterium. As this can influence assay outcome, we recommend testing several strains. See our preprint: www.biorxiv.org/content/10.1...
Cytokinin-mediated trichome initiation in Nicotiana benthamiana upon Agrobacterium tumefaciens infiltrationInfiltration of Agrobacterium tumefaciens in leaves of Nicotiana benthamiana has become a standard method for transient expression in plants. Like many Solanaceae species, N. benthamiana develops on i...www.biorxiv.org